5x Tris-Glycine Buffer

Tris-Glycine Buffer is intended for use as the running buffer in SDS-PAGE of proteins. it is the most commonly used as the electrophoresis buffer during the stacking and resolve process of SDS-PAGE.

The buffer consist Tris Base, Glycine & SDS in recommended concentrations. The buffer is supplied in 5x concentrate and should be diluted prior to use in electrophoresis. The components of 5x Tris-Glycine running buffer are 125mM Tris; 1.25M Glycine; 0.5% (W/V) SDS.