Free Hemoglobin Content Assay kit

Red blood cells rupture and free hemoglobin (FHb) content increases in the plasmawhenhemolysis occurs in blood vessels. Plasma FHb concentration increases mildly to moderatelyinpatients with autoimmune hemolytic anemia, sickle cell disease or thalassemia. The patientshavehematuria symptoms who suffer from paroxysmal nocturnal hemoglobinuria, paroxysmal coldhemoglobinuria or unstable hemoglobin disease. It is important to detect FHb content for diagnosisof hemolytic diseases. Heme in hemoglobin has the catalytic function of peroxidase analogues. It could catalyzetheoxidation of 4-aminoantipyrine and phenyl amines by H2O2 to form purple quinones. It hasacharacteristic absorption peak at 546 nm, and its color depth is directly proportional to FHbcontent.